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M94A2328.TXT
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1994-10-25
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Document 2328
DOCN M94A2328
TI Prevalence of antibodies to herpes simplex virus in saliva among
Japanese female bath-house workers.
DT 9412
AU Ichikawa S; Tsukano K; Okumura S; Zhang ZQ; Kihara M; Toba M; Soda K;
Dept. of Public Health, Yokohama City Univ. School of Medicine,; Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):329 (abstract no. PC0252). Unique
Identifier : AIDSLINE ICA10/94370247
AB OBJECTIVES: We established a sensitive ELISA method (ABC-ELISA) to
detect the antibodies against herpes simplex virus (HSV) in saliva, and
observed the prevalence among female bath-house workers. MATERIALS &
METHODS: Paired specimens of serum and saliva were collected from 138
healthy nurse students. Salivary specimens were collected from 191
female bath-house workers. After centrifugation, clear saliva were
collected and stored at 40 degrees C until testing. Serum and saliva
were tested with ABC-ELISA method by using kits of avidin-biotin complex
(VECTASTAIN) for detection of antibodies to HSV. RESULTS & DISCUSSION:
All saliva specimens from bath-house workers were negative in ELISA test
(ELAVIA MIXT) for anti-HIV. But, the prevalence of HSV antibody in
saliva among this population was 84.3% (161/191). The positive rates of
HSV antibody by age were 65% in the 20-29 age group, 89% in the 30-39
age group and 94% in the > or = 40 age group. The prevalence of HSV
antibody in saliva among the youngest bath-house worker group was
significantly higher than that of nurse students (43.5%). Collection of
blood specimens in epidemiological surveillance is not easy. Though it
is necessary to improve sensitivity of ABC-ELISA method for testing of
saliva, the mentioned results suggested that saliva was useful for
detection of antibodies to HSV in epidemiological studies.
DE Adult Antibodies, Viral/*ANALYSIS *Enzyme-Linked Immunosorbent Assay
Female Herpes Simplex/*EPIDEMIOLOGY Human HIV Antibodies/ANALYSIS
Japan/EPIDEMIOLOGY *Mass Screening Population Surveillance
Prostitution/*STATISTICS & NUMER DATA Saliva/*IMMUNOLOGY
Simplexvirus/*IMMUNOLOGY MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).